Health care Essay Example | Topics and Well Written Essays - 500 words - 4

Health care - Essay Example The caregivers were even in favor of such services because they needed counseling services due to the every day stress they were experiencing and this stress was ruining their work and personal life balance. According to a research conducted by Roberts and others, people who are near to death, stay at home and prepare to die and need the support of caregivers such as health care professionals and family members to live the remaining days of their life in peace (Roberts 2007). With the assistance of telecommunication, assistance can be provided to such patients as healthcare settings can not remain available 24/7. Those who are terminally ill can not move themselves and require immediate attention when they suffer different kinds of health issues. Availability of tele hospice services will assist such patients as they will be able to obtain advice and treatment for their issues over a simple phone call. Several patients suffer from loneliness and anxiety in their every day life, these disorders are mostly found in those individuals who do not have support and who do not have someone to talk to. It is not easy for patients to reach hospitals all the time when they need support and assistance from care givers. The health care professionals even need assistance and counseling because of the traumatic events they experience in their every day life. Tele hospice services can help solve all these issues. Due to availability of hospice services through the telecommunication, patients who do not need to attend health care settings can easily obtain support and counseling services over phone calls. On the other end, a special line for health care professionals can help them release their stress and obtain advice when they suffer from heavy stress due to their everyday work. The health care settings experience the problem of limited staff availability due to which they can not attend all pat ients and those patients who do not need to

Investment Environment Assignment Example | Topics and Well Written Essays - 3500 words - 1

Investment Environment - Assignment Example The company wants to enter the market of Saudi Arabia. In this context, the market of Saudi Arabia will be analyzed with respect to different parameters. The analysis of the market will help to bring out the potential and threats of the market for the international business operations. This report will assist to provide details about marketing opportunities and potentials for the global business environment. Argos Ltd is the UK based retail firm that operates as a multi-channel retailer recognized for delivering value, choice, and convenience to the customers. The company offers general merchandise and products for home through their 700 stores located in the UK market and Republic of Ireland along with online and telephone assistance. During the financial year of 2010, the company had been able to generate more than  £4.30 billion with the workforce of 33,000 across the business segments. The company serves more than 130 million customers annually. They are able to generate sales through the internet that consists of 26% of the total sales. These are few of the reasons for selecting the company for the analysis (Argos Limited, 2011). The Fast Moving Consumable Goods (FMCG) segment of Saudi Arabia shows huge potential. There are more than 24.20 million people out of which 7.0 million are non-Saudi residents consuming FMCG goods (Mousa, 2009). The retail sector of Saudi Arabia is expected to grow by a whopping US$ 125 billion by 2014 as there are several international brands planning to enter the retail market of Saudi Arabia. In the global retail ranking, Saudi Arabia has positioned itself in the ninth place. The market has been able to attract many international retailers and had overtaken well-known retail destinations such as Russia, Hong Kong, and Japan. More than 43% of international brands are attracted towards this retail destination.  

Introduction to programming Essay Example for Free

Introduction to programming Essay As this course is titled â€Å"Introduction to programming†, therefore it is most essential and appropriate to understand what programming really means. Let us first see a widely known definition of programming. Definition: A program is a precise sequence of steps to solve a particular problem.† It means that when we say that we have a program, it actually mean that we know about a complete set activities to be performed in a particular order. The purpose of these activities is to solve a given problem. Alan Perlis, a professor at Yale University, says: See more: how to write an introduction paragraph for an essay It goes against the grain of modern education to teach children to program. What fun is there in making plans, acquiring discipline in organizing thoughts, devoting attention to detail and learning to be self-critical? It is a sarcastic statement about modern education, and it means that the modern education is not developing critical skills like planning, organizing and paying attention to detail. Practically, in our day to day lives we are constantly planning, organizing and paying attention to fine details (if we want our plans to succeed). And it is also fun to do these activities. For example, for a picnic trip we plan where to go, what to wear, what to take for lunch, organize travel details and have a good time while doing so. When we talk about computer programming then as Mr. Steve Summit puts it â€Å"At its most basic level, programming a computer simply means telling it what to do, and this vapid-sounding definition is not even a joke. There are no other truly fundamental aspects of computer programming; everything else we talk about will simply be the details of a particular, usually artificial, mechanism for telling a computer what to do. Sometimes these mechanisms are chosen because they have been found to be convenient for programmers (people) to use; other times they have been chosen because theyre easy for the computer to understand. The first hard thing about programming is to learn, become comfortable with, and accept these artificial mechanisms, whether they make sense to you or not. â€Å" Why Programming is important The question most of the people ask is why should we learn to program when there are so many application software and code generators available to do the task for us. Well the answer is as give by the Matthias Felleisen in the book ‘How to design programs’ â€Å"The answer consists of two parts. First, it is indeed true that traditional forms of programming are useful for just a few people. But, programming as we the authors understand it is useful for everyone: the administrative secretary who uses spreadsheets as well as the high-tech programmer. In other words, we have a broader notion of programming in mind than the traditional one. We explain our notion in a moment. Second, we teach our idea of programming with a technology that is based on the principle of minimal intrusion. Hence, our notion of programming teaches problem-analysis and problem-solving skills without imposing the overhead of traditional programming notations and tools.† Hence learning to program is important because it develops analytical and problem solving abilities. It is a creative activity and provides us a mean to express abstract ideas. Thus programming is fun and is much more than a vocational skill. By designing programs, we learn many skills that are important for all professions. These skills can be summarized as: Critical reading Analytical thinking Creative synthesis What skills are needed Programming is an important activity as people life and living depends on the programs one make. Hence while programming one should Paying attention to detail Think about the reusability. Think about user interface Understand the fact the computers are stupid Comment the code liberally Paying attention to detail In programming, the details matter. This is a very important skill. A good programmer always analyzes the problem statement very carefully and in detail. You should pay attention to all the aspects of the problem. You cant be vague. You cant describe your program 3/4th of the way, then say, You know what I mean?, and have the compiler figure out the rest. Furthermore you should pay attention to the calculations involved in the program, its flow, and most importantly, the logic of the program. Sometimes, a grammatically correct sentence does not make any sense. For example, here is a verse from poem Through the Looking Glass written by Lewis Carol: â€Å"Twas brillig, and the slithy toves Did gyre and gimble in the wabe â€Å" The grammar is correct but there is no meaning. Similarly, the sentence, Mr. ABC sleeps thirty hours every day, is grammatically correct but it is illogical. So it may happen that a program is grammatically correct. It compiles and runs but produces incorrect or absurd results and does not solve the problem. It is very important to pay attention to the logic of the program. Think about the reusability When ever you are writing a program, always keep in mind that it could be reused at some other time. Also, try to write in a way that it can be used to solve some other related problem. A classic example of this is: Suppose we have to calculate the area of a given circle. We know the area of a circle is (Pi * r2). Now we have written a program which calculates the area of a circle with given radius. At some later time we are given a problem to find out the area of a ring. The area of the ring can be calculated by subtracting the area of outer circle from the area of the inner circle. Hence we can use the program that calculates the area of a circle to calculate the area of the ring. Think about Good user interface As programmers, we assume that computer users know a lot of things, this is a big mistake. So never assume that the user of your program is computer literate. Always provide an easy to understand and easy to use interface that is self explanatory. Understand the fact that computers are stupid Computers are incredibly stupid. They do exactly what you tell them to do: no more, no less unlike human beings. Computers cant think by themselves. In this sense, they differ from human beings. For example, if someone asks you, â€Å"What is the time?†, â€Å"Time please?† or just, â€Å"Time?† you understand anyway that he is asking the time but computer is different. Instructions to the computer should be explicitly stated. Computer will tell you the time only if you ask it in the way you have programmed it. When youre programming, it helps to be able to think as stupidly as the computer does, so that you are in the right frame of mind for specifying everything in minute detail, and not assuming that the right thing will happen by itself. Comment the code liberally Always comment the code liberally. The comment statements do not affect the performance of the program as these are ignored by the compiler and do not take any memory in the computer. Comments are used to explain the functioning of the programs. It helps the other programmers as well as the creator of the program to understand the code. Program design recipe In order to design a program effectively and properly we must have a recipe to follow. In the book name ‘How to design programs’ by Matthias Felleisen.and the co-worker, the idea of design recipe has been stated very elegenlty as â€Å"Learning to design programs is like learning to play soccer. A player must learn to trap a ball, to dribble with a ball, to pass, and to shoot a ball. Once the player knows those basic skills, the next goals are to learn to play a position, to play certain strategies, to choose among feasible strategies, and, on occasion, to create variations of a strategy because none fits. â€Å"

High Performance Liquid Chromatography Experiment

High Performance Liquid Chromatography Experiment INTRODUCTION Pharmaceutical Analysis may be defined as the application of analytical procedures used to determine the purity, safety and quality of drugs and chemicals. The term Pharmaceutical analysis is otherwise called quantitative pharmaceutical chemistry. Pharmaceutical analysis includes both qualitative and quantitative analysis of drugs and pharmaceutical substances starts from bulk drugs to the finished dosage forms. In the modern practice of medicine, the analytical methods are used in the analysis of chemical constituents found in human body whose altered concentrations during disease states serve as diagnostic aids and also used to analyze the medical agents and their metabolites found in biological system. Qualitative inorganic analysis seeks to establish the presence of given element or inorganic compound in a sample. Qualitative organic analysis seeks to establish the presence of a given functional group or organic compound in a sample. Quantitative Quantitative analysis seeks to establish the amount of a given element or compound in a sample. The term quality as applied to a drug product has been defined as the sum of all factors, which contribute directly or indirectly to the safety, effectiveness and reliability of the product. These properties are built into drug products through research and during process by procedures collectively referred to as Quality control. Quality control guarantees with in reasonable limits that a drug products Is free of impurities. Is physically and chemically stable Contains the amount of active ingredients as stated on the label and Provides optimal release of active ingredients when the product is administered. Most modern analytical chemistry is categorized by two different approaches such as analytical targets or analytical methods. INTRODUCTION FOR CHROMATOGRAPHY: High performance liquid chromatography is the process, which seperates mixture containing two or more components under high pressure. In this the stationary phase is packed in column one end of which is attached to a source of pressurized liquid mobile phase. High performance liquid chromatography is the fasted growing analytical technique for the analysis of drug. Its simplicity, high specificity and wide range of sensitivity makes its ideal for the analysis of many drugs in both dosage form and biologic fluids. HPLC is also known as High performance liquid chromatography. It is essential form column chromatography in which the stationary phase is consists of a small particles (3-5o µm) packing contained in a column with a small bore (2-5mm), one end of which is attached to source of pressurized liquid eluent(mobile phase). Different Types of Principles: According to the phases involved, HPLC can be classified into several types, which are as follows: Normal Phase Chromatography (NPC) Reverse Phase Chromatography (RPC) Liquid Solid Chromatography or adsorption HPLC Liquid Liquid Chromatography or Partition HPLC Ion exchange Chromatography or Ion exchange HPLC Size exclusion or gel permeation or steric exclusion HPLC 1. Normal Phase Chromatography (NPC): In normal phase chromatography, the stationary phase is more polar then the mobile phase, and the mobile phase is a mixture of organic solvents with out added water (e.g. isopropane with hexane) and the column packing is either an inorganic adsorbent (silica) are a polar bonded phase (cyanno, diol, amino) on a silica support. Sample retention in normal phase chromatography increases with the polarity of mobile phase decreases. They are eluted in the order of increasing polarities. 2. Reverse Phase Chromatography (RPC): In reverse-phase chromatography, the stationary phase is less polar than the mobile phase and the mobile phase is a mixture of organic and aqueous phase. Reverse-phase chromatography is typically more convenient and rugged than the other forms of liquid chromatography and is more likely to result in a satisfactory final separation. High performance RPC columns are efficient, stable and reproducible. In this, the solutes are eluted in the order of their decreasing polarities. These are prepared by treating the surface silanol group of site with an organic chloro silane reagent. INSTRUMENTATION: RECORDER SCHEMATIC DIAGRAM OF HPLC a. Pumps: Pumps are required to deliver a constant flow of mobile phase at pressures ranging from 1 550 bar pumps capable of pressure up to 6000 psi provide a wide range of flow rates of mobile phase, typically from 0.01-10ml min-1. Low flow rates (10-100à ¯Ã‚ Ã‚ ­l min-1) are used with micro bore columns, intermediate flow rates (0.5-2ml min-1) are used with conventional analytical HPLC columns, and fast flow rates are used for preparative or semi preparative columns and for slurry packing techniques. Mechanical pumps of the reciprocating piston type view a pulsating supply of mobile phase. A damping device is there fore required to smooth out the pulses so that excessive noise at high levels of sensitivity or low pressure does not detract from detection of small quantities of sample. This type of pump is mostly used. Dual piston reciprocating pumps produce an almost pulse free flow because the two pistons are carefully faced so that as one is filling the other is pumping. These pumps are more expensive than single piston pumps but are of benefit when using a flow sensitive detector such as ultraviolet or refractive index detector. b. Injection Systems: Injection ports are of two basic types, (A) those in which the sample with injected directly into the column and (B) those in which the sample is deposited before the column inlet and then swept by a valving action into the column by the mobile phase. c. Columns: HPLC columns are made of high quality stainless steel, polish internally to a mirror finish. Standard analytical columns are 4-5 mm internal diameter and 10-30 cm in length. Shorted columns (3-6 cm) containing a smaller particles size packing material (3 or 5 à ¯Ã‚ Ã‚ ­m) produce similar or better efficiencies, in terms of the number of theoretical plates (about 7000), that those of 20 cm columns containing 10 à ¯Ã‚ Ã‚ ­m irregular particles and are used an short analysis time and highest throughput of samples are required. Micro bore columns of 1-2 mm internal diameter and 10-25 cm in length have certain advantages of lower detection limits and lower consumption of solvent, the latter being important if expensive HPLC grade solvents are used. HPLC are also being carried out on the semi preparative scales by using columns of 7-10 mm or 20-40 mm internal diameter respectively. d. Detectors: The most widely used detectors for liquid chromatography are Detector Analytes Solvent Requirements Comments UV-Visible Any with chromophores UV-grade non UV absorbing solvents Has degree of selectivity and useful for many HPLC applications Fluorescence Fluorescent compounds UV-grade non UV absorbing solvents Highly selective and sensitive, often used to analyze derivitized compounds Refractive index Compounds with different RI than mobile phase Cannot run mobile phase gradients Limited sensitivity Conductivity Charged or polar compounds Mobile phase must be conducting Excellent for ion exchange compounds Electrochemical Readily oxidized or reduced compounds, specially biological samples Mobile phase must be conducting Very selective and sensitive Mass-Spectrometer Broad range compounds Must use volatile solvents or volatile buffers Highly sensitive. Many modes available. Needs trained person Theoretical principles of HPLC: a. Retention time: The time is required between the injection point and the peak maximum is called the retention time. It is denoted as the Rt. It is mainly useful for the qualitative analysis for the identification of compound. b. Capacity factor: It represents the molar ratio of the compound in the stationary phase and the mobile phase. It is independent of column length and mobile phase flow rate. It is denoted as the k. It should be kept 1-10. If k values are too low it is likely that the solutes may be adequately resolved and for high k values the analysis time is too long. It can be calculated by tr t0 k = - t0 tr = Retention time, t0 = Dead time. c. Tailing factor: Closer study of a chromatographic show that the Gaussian forms is usually not completely symmetrical. The graph spread out to a greater or lesser extent, forming a tail. It reduces the column plate number which intern influences the resolution. Tailing is mainly due to deteriorated column, overloading column, extra column-volumes, and incompatibility of sample with standard and/or mobile phase. Practically it can be calculated or determined at 10% of the total peak height. It must not be greater than 2.0 d. Resolution: The degree of separation of one component from another is described by the resolution. It is generally denoted by Rs. It is measured as the difference in retention time and the arithmetic mean of the two peak widths. tr2 tr1 Rs = 0.5(w1 + w2) tr2 = Retention time of first peak w1 = width of first peak tr1 = Retention time of second peak w2 = width of second peak e. Theoretical plates: It is important property of the column. It reflects its quality of separation and its ability to produce sharp, narrow peak and achieving good resolution of peak. N denotes it. 3500 X L (cm) Theoretical plates = - dp( µm) L = length of the column in cm, dp = diameter of the particle ( µm) It follows that if the exchange is fast and efficient, the theoretical plate will be small in size and there will be large number of plates in the column. f. Height equivalent to theoretical plate (HETP): Number of plates directly proportional to the column length (L) and inversely proportional to the diameter of the particles (dp). The value of H is a criterion for the quality of a column. Lower the HETP, higher is the efficiency of the column. Its value depends upon particle size, flow rate, viscosity of mobile phase. H = L/N L = Length of column, N = No. of theoretical plate HPLC method development: The wide variety of equipment, columns, eluent and operational parameters involved makes high performance liquid chromatography (HPLC) method development seem complex. The main objective of method development is to obtain a good separation with minimum time and effort. Based on the goal of separation, the method development is preceded. The steps involved are: Information on sample, define separation goals Need for special HPLC procedure, sample pretreatment, etc. Choose detector and detector settings Choose LC method, preliminary run; Estimate best separation conditions Optimize separation conditions Check for problems or requirement for special procedure Validation for release to routine laboratory The following must be considered when developing an HPLC method: Keep it simple Try the most common columns and stationary phases first Thoroughly investigate binary mobile phases before going on to ternary Think of the factors that are likely to be significant in achieving the desired resolution. Mobile phase composition, for example, is the most powerful way of optimizing selectivity whereas temperature has a minor effect and would only achieve small selectivity changes. pH will only significantly affect the retention of weak acids and bases. VALIDATION OF ANALYTICAL METHOD IN PHARMACEUTICAL ANALYSIS: Validation is documented evidence, which is completed to ensure that an analytical method is accurate, reproducible and robust over the specific range. The quality of the analytical data is a key factor in the success of a drug development program. The process of method development and validation has a direct impact on the quality of these data. Method validation: Method validation is the process to confirm that analytical procedure employed for a specific test is suitable for its intended use. Method needs to be validated or revalidated Before their introduction into routine use Whenever the conditions changes for which the method has been validated , e.g., instrument with different characteristics Whenever the method is changed, and the change is outside the original scope of the method. Depending on the use of the assay, different parameters will have to be measured during the assay validation. ICH and several regulatory bodies and Pharmacopoeia have published information on the validation of analytical procedures METHOD VALIDATION PARAMETERS: SPECIFICITY. ACCURACY. PRECISION. LINEARITY. ROBUSTNESS. SOLUTION STABILITY. The goal of the validation process is to challenge the method and determine the limit of allowed variability for the conditions needed to run the method. The following statistical parameters are to be determined to validate the developed method. Correlation coefficient(r): When the changes in one variable are associated or followed by changes in the other, it is called correlation. The numerical measure of correlation is called the coefficient of correlation and is defined by the relation. à ¯Ã‚ Ã¢â‚¬Å" (x x) (y -y) r = à ¢Ã‹â€ Ã… ¡ à ¯Ã‚ Ã¢â‚¬Å"(x -x) 2 à ¯Ã‚ Ã¢â‚¬Å"(y -y Regression equation: Regression equation= I + aC Y2 Y1 a = slope = X2 X1 I = Intercept = regression a C As a percentage of mean absorbance. 3. Standard Deviation: S = à ¢Ã‹â€ Ã… ¡ à ¯Ã‚ Ã¢â‚¬Å" (X- X!) 2/N 1 Where, X = observed values X! = Arithmetic mean = à ¯Ã‚ Ã¢â‚¬Å"X/N N = Number of deviations For practical interpretation it is more convenient to express S in terms of percent of the approximate average of the range of analysis is used in the calculation of S. This is called co-efficient of variation (C.V) or percent relative standard deviation (%RSD). C.V OR %RSD = 100* S/ X! Criteria for Validation of the Method CHARACTERISTICS ACCEPTABLE RANGE Specificity No Interference Accuracy Recovery (98-102%) Precision RSD Linearity Correlation Coefficient(r)>0.99 Range 80-120% Stability >24h or >12h DRUG PROFILE RIZATRIPTAN BENZOATE: Structure: Chemical name : N,N diethyl -5-(1H-1,2,4-triazol-1-1-ylmetyl)-1H Indole-3 Ethanamine monobenzoate Molecular Formula : C15H19N5.C6H5COOH Molecular weight : 391.47 Description: White crystalline powder Melting point: 178-1800C Solubility: Sparingly soluble in water and methanol Storage: Air tight container protect from light. Drug Category: Anti migraine drug THERAPEUTIC RATIONAL RIZATRIPTAN BENZOATE: CLINICAL PHARMACOLOGY: Mechanism of action: Rizatriptan binds with high affinity to human 5-HTIB and 5-HTID receptors leading to cranial blood vessel constriction. Pharmacokinetics: Absorption: Completely absorbed from GI tract, absolute bioavailability is 45% plasma peak concentration attained with in 1-1.5 hours (conventional tablet )or 1.6-2.5 hours (orally disintegrating tablet)after oral administration. Distribution: Crosses placenta and is distributed in to milk in animal, no studies in pregnant or nursing women. Metabolism: Metabolized principally via oxidative deamination by Mao-A to an inactive indole acetic acid metabolite Elimination: Excreted principally in urine(14% of dose as unchanged drug and 51 % a indole acetic acid metabolite Adverse effects: Dry mouth Dizziness Pain tightness/pressure in neck/throat/jaw. Nausea Chest pain Parasthesia Fatigue Dosage and administration: The dose range of Rizatriptan benzoate is 10-30mg orally once daily.Rizatriptan benzoate can be administer orally disintegrating tablet with out meals. LITERATURE REVIEW Sasmitha Kumar et al: has been developed UV spectroscopic method for estimation of Rizatriptan benzoate.The drug shows maximum absorption at 277 nm and 281 nm and obeys beer-lamberts law in the concentration of 0.5-20  µg/ml at 277 nm and 0.5-80  µg/ml at 281 nm respectively. The percentage recovery was found to be 97-100%. Madhukar et al; has been developed reverse phase high performance liquid chromatographic method for determination of Rizatriptan benzoate. The proposed method utilized column L1 inertsil ODS-3v, 250 nmx4.6 mm having particle size, 5 µm. The mobile phases were comprised of A, B of Acetonitrile and buffer pH 6.5 at UV detection 225 nm.The method shows recovery 96.64-97.71 Sachin jagthap et al; has been developed stability indicating reversed phase high performance liquid chromatographic method for the determination of Rizatriptan benzoate in bulk powder and in pharmaceutical formulations. The method utilizes c18 column having dimension 250mmx4.6 mm having particle size,5.0  µm using a mobile phase 0.01M sodium dihydrogen phosphate buffer: Methanol , at a flow rate 1ml/min at ambient temperature and detected at 225 nm.and the method was validated according to ICH guidelines Quizi zhang et al: has been developed, a high performance liquid chromatographic method for the determination of Rizatriptan benzoate in human plasma.using asingle step liqid liqid extraction with metyl tertiary butyl ether, the analytes separated usig amobile phase consisting of 0.05%v/v triehylamine in water adjusting ph 2.75 with 85% phosphoric acid and acetonitrile.fluroscence detection was performed at an excitation wavelength of 225 nm and an emission wavelength of 360 nm.The linearity for rizatriptan was within the concentration range of 0.5-50ng/ml. Rajendra Kumar et al: has been developed and validated stability a stability indicating high performance liquid chromatographic method for Rizatriptan benzoate.The force degradation studies were performed on bulk sample of Rizatriptan benzoate. The method utilizes a zorbax SB-CN column with dimension of 250 mmx4.6 mm, 5um column. The mobile phase consists of a mixture of aqueous potassium dihydrogen ortho phosphate (ph3.4), acetonitrile and methanol. Rauza bagh et al: has been developed a spectroscopic method for analysis of Rizatriptan benzoate in bulk and tablet dosage form. The Rizatriptan benzoate shows maximum absorbance at 225 nm. Beers law was obeyed in the concentration range of 1-10 µg/ml. AIM AND PLAN OF WORK The present aim is to develop a new simple and rapid analytical method to estimate the Rizatriptan benzoate The plan of the proposed work includes the following steps: To undertake solubility studies for analytical studies of Rosuvastatin calcium Develop initial chromatographic conditions. Setting up of initial chromatographic conditions for the assay of Rosuvastatin calcium Optimization of initial chromatographic conditions. Validation of the developed HPLC Analytical method according to ICH method validation parameters. EXPERIMENTAL NEW RP-HPLC METHOD FOR THE ESTIMATION OF RIZATRIPTAN BENZOATE IN TABLET DOSAGE FORM A simple reverse phase HPLC methods was developed for the determination of Rizatriptan benzoate in tablet dosage form. Zorbax Eclipse XBD C18 (250 cm ÃÆ'- 4.6 mm) column in isocratic mode with mobile phase Buffer ph 5.0: Methanol (80:20) was used and pH-3 adjusted with tri ethylamine. The flow rate was 1.0 ml/min and UV detection at 225nm. The retention time 3.0 min. The proposed method was also validated. EXPERIMENTAL 1. Instrumentation: Shimadzu LC-10A HPLC Vacuum pump Gelmon science Elico SL-164 double beam UV-Visible spectrophotometer Ultra sonicator 3.5L 100(pci) 2. Chemicals: Water HPLC grade Methanol HPLC grade (Merck) Potassium dihydrogen orthophosphate(AR Grade) Triethylamine (AR Grade) 5.1 OPTIMIZATION: 1. Selection of wavelength: After solubility study for the drug solvent was selected and appropriate concentration of Rizatriptan benzoate standards with solvent were prepared. The solution were then scanned by using doubl beam UV-Visible spectrophotometer the range between 200-400nm.The overlain spectra for the both drug were observed and maximum wavelength was finally selected. 2. Selection of mobile phase: To develop a prà ©cised and robust HPLC method for determination of Rizatriptan benzoate , its standard solution were injected in the HPLC system. After literature survey and solubility data different composition of mobile phase of different flow rates were employed in order to determine the best condition for effective separation of drugs. 3. Selection of column: Initially different C8 and C18 columns were tried for selected composition of mobile phase and quality of peaks were observed for the drugs. Finally the column was fixed upon the satisfactory results of various system suitability parameters such as column efficiency, retention time, tailing factor / peak asymmetry of the peaks. Other parameters such as flow rate, column temperature etc. were selected by varying its value up to certain levels and results were observed. The value at satisfactory results were obtained has been selected for the method. The final selection of chromatographic conditions as follows Optimized chromatographic conditions Preparation of Buffer ph 5.0: Dissove 2.76 gm of potassium dihydrogen orthophosphate in 1000ml of HPLC water plus 5.0 mlof Triethylamine. Mix and adjust PH 5.0 with orthophosporic acid. Filter with 0.45u nylon filter. Preparation of mobile phase: The mobile phase was prepared by mixing Buffer: Methanol (80:20). the solution was then filtered through 0.45ÃŽÂ ¼m membrane filter and sonicated. Preparation of standard stock solution: Standard solution of the pure drug was prepared by dissolving 73.0 mg of Rizatriptan benzoate in 100ml volumetric flask. The drugs were dissolved by using mobile phase as a diluent. Add about 50ml of diluent and sonicate to dissolve. Make up the volume with diluent. Mix well. Further dilute 5.0ml of the above solution to 250ml with diluent, mix well. Preparation of sample solution: Weight and transfer 10 intact tablet in into a100ml volumetric flask. Add about 50ml of diluent and sonicate for 15 min and make up the volume with diluent. Mix well, filter through 25 mm 0.45 u nylon , discard 4ml filtrate. Further dilute 5ml of the solution to 250 ml with diluent and mix well. CONCLUSION The evaluation of obtained values suggests that the proposed HPLC methods provide simple, precise, rapid and robust quantitative analytical method for determination of Rizatriptan benzoate in tablet dosage form. The mobile phase is simple to prepare and economical. After validating proposed method as per ICH guidelines and correlating obtained values with the standard values, satisfactory results were obtained. Hence, the method can be easily and conveniently adopted for routine estimation of Rizatriptan benzoate in tablet dosage form.

Fahrenheit 451 by Ray Bradbury :: essays research papers

Fahrenheit 451  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚     Ã‚  Ã‚  Ã‚  Ã‚  The novel Fahrenheit 451 was wrote by Ray Bradbury. The setting takes place in future times. The main character (protagonist) in Fahrenheit 451 is, ‘Guy Montag’. Guy Montag has been a fireman for ten years and he doesn’t realize that he is not joyful towards his life. He never questioned the joy of midnight runs. The plot of the story is basically how Guy turned from being an ignorant person into being a person filled with intelligence and a new outlook on life. Guy is a normal man that can’t find his true happiness within. 451 degrees is the temperature that books burn. Literature is taboo in this futuristic society, and Guy’s job is to burn any books or news clippings he sees, however his views change for the better further in the novel.   Ã‚  Ã‚  Ã‚  Ã‚  The author Ray Bradbury created a world where literature is not allowed. He’s trying to show how bad our world would be without writings and poetry. Guy is one of the many firemen who are supposed to burn every book or writing that they see. People caught with books in their house were forced out of the house while the firemen would burn the house down. The climax of the story is when Guy meets a seventeen-year-old professor who brings the life of writings back into vision. Guy soon realizes that he is unhappy, because he can’t express himself through reading novels and most of all poetry. â€Å"†¦And Guy Montag the same person suddenly realized what he had to do†(Bradbury 89) this is the climax of the story because; Guy realizes that he doesn’t want to live in a world without Literature.   Ã‚  Ã‚  Ã‚  Ã‚  The best scene in the novel was when Guy met his old professor Faber for the first time in thirty years. Faber impounded the thoughts and beauty of Literature into Guy’s head making him a more out going person. This was truly the time when Guy realized that he use to love reading. Faber would read some poems to Guy, and Guy loved every minute of it. Guy was seeing things in life much more beautiful through poems. Faber taught guy to look at the moon and the clouds as beauty. This was the final turning point in Guy’s life. â€Å"I haven’t read writings to anyone for forty years ago when schools were starting to die off.†(Faber said to guy)   Ã‚  Ã‚  Ã‚  Ã‚  I can’t understand how these people in the future of this novel were able to give up reading.

To Autumn by John Keats Essay -- To Autumn John Keats Poetry Essays

To Autumn by John Keats â€Å"To Autumn† is one of the most famous, and perfect odes written by John Keats, and any modern writer. It is quite fitting that his greatest piece was the last one that he ever wrote before he met with his unfortunate end. However, this ode has some significant differences to the other odes that he has written. Firstly, there is no flight from reality, or deviation into imagination or dream, in fact there is no narrative voice at all. Secondly, it has an unprecedented emphasis and commemoration of change and progress, not only through autumn, but through all mortal events. While the title implies a progression through autumn, the ode also has references to an aging day, and even personal maturity. The first stanza is brimming with specific vivid visual imagery. The first which relates to the change in the season and day is the â€Å"maturing sun.† This sun makes the fruit ripen and cause the burst of ripe food for harvesting. He then goes on to describe the outburst of ripening fruit to an excruciating intensity. The apples are so plentiful that... To Autumn by John Keats Essay -- To Autumn John Keats Poetry Essays To Autumn by John Keats â€Å"To Autumn† is one of the most famous, and perfect odes written by John Keats, and any modern writer. It is quite fitting that his greatest piece was the last one that he ever wrote before he met with his unfortunate end. However, this ode has some significant differences to the other odes that he has written. Firstly, there is no flight from reality, or deviation into imagination or dream, in fact there is no narrative voice at all. Secondly, it has an unprecedented emphasis and commemoration of change and progress, not only through autumn, but through all mortal events. While the title implies a progression through autumn, the ode also has references to an aging day, and even personal maturity. The first stanza is brimming with specific vivid visual imagery. The first which relates to the change in the season and day is the â€Å"maturing sun.† This sun makes the fruit ripen and cause the burst of ripe food for harvesting. He then goes on to describe the outburst of ripening fruit to an excruciating intensity. The apples are so plentiful that...

Exposé of Hypocrisy of Imperialism in Conrad’s Heart of Darkness Essay

In his novella Heart of Darkness, author Joseph Conrad attempts to expose the hypocrisy of imperialism since its explorers emphasize the importance of helping natives, while actively exploiting them. He also depicts the Africans in the story as nothing more than mindless savages. During his journey to the Congo, Conrad witnessed the horrors firsthand that the Europeans inflicted upon the African natives. He shares his experiences through Marlow, the protagonist, who sees the evil side of humanity through its materialism. Conrad denounces the oppressive nature of those of his time by exposing their social darwinist thought. Imperialism is portrayed as a hypocritical tool used purely for the Europeans’ own benefit. Europeans began what is known as â€Å"the scramble for Africa† in the late 19th century. The major powers all fought each other to gain control, disregarding the Africans in the process. The Europeans believed themselves to be taking up â€Å"the white man’s burden†, which was the belief that the Europeans had a duty to gain control of Africa and civilize the inhabitants in order to help them. The hypocrisy of it was that what they deemed as helping and civilizing them was actually entirely against the natives’ wishes, as their culture was being destroyed as well as their people enslaved. Conrad depicts this throughout his novel, Conrad denounces oppression by showing racism to criticize the immorality and cruelty of enslaving others for personal gain. Inhumane treatment of the africans is expressed through how they wore rags, which gives a sense of desolation. Racism is conveyed through the dehumanization of the natives, revealing that their captors went as far to put them in collars, showing they were seen as and treated like dogs. Even Marlow refers to his helmsman as a piece of machinery. Nigerian writer Chinua Achebe, author of Things Fall Apart, famously criticized Heart of Darkness in his 1975 lecture An Image of Africa: Racism in Conrad’s â€Å"Heart of Darkness†, saying the novella de-humanized Africans, denied them language and culture and reduced them to a metaphorical extension of the dark and dangerous jungle into which the Europeans venture. All the European colonists desired was wealth and treasures, willing to go as far as completely exploit other human beings. The metaphor comparing colonists to burglars was used to convey that they had no morals and did whatever they please in order to achieve personal gain, including exploiting them. This is all very hypocritical considering they believed themselves to be helping and civilizing them. Conrad uses Mr. Kurtz to unveil how he feels about imperialism as a whole. Marlow describes how Mr. Kurtz says everything is his own, which shows that imperialists claimed Africa is theirs and it was okay to exploit the natives as slaves. Conrad uses the dehumanization of Kurtz to exemplify his beliefs to maintain one’s morals to avoid becoming caught up in a world of materialistic desires. Mr. Kurtz is described as having a bald head similar to that of an ivory ball, leading the reader to believe that all he thinks about is ivory. The fact that he is withered is to show Conrad’s belief of what greed will result in upon a person.